Kazusa:RhizoBase:Bradyrhizobium japonicum USDA110

出典: KazusaWiki

About Bradyrhizobium japonicum and RhizoBase

Bradyrhizobium japonicum, a member of rhizobia, has an ability to form root nodules on soybeans (Glycine max). B. japonicum strain USDA110, which was originally isolated from soybean nodule in Florida, USA in 1957, has been widely used for the purpose of molecular genetics, physiology, and ecology, because this strain is superior at symbiotic nitrogen fixation with soybeans as compared with other strains.

Bradyrhizobium japonicum (in NCBI TAXONOMY BROWSER)

We initiated the genome analysis of B. japonicum strain USDA110 in 2001, and the complete nucleotide sequence of its genome was determined in December 2002. The genome of B. japonicum is a single circular chromosome of 9,105,828 bp long with an average GC content of 64.1%. The chromosome comprises 8317 potential protein-coding genes, one set of rRNA genes and 44 tRNA genes.

RhizoBase provides an easy way of accessing the sequcence and all-inclusive annotation data through image maps, keyword searches and the gene category list. This database has been developed and maintained by Yasukazu Nakamura, Takakazu Kaneko and Satoshi Tabata at Kazusa DNA Research Institute.

Derivatives

Strain Genetype or Phenotye Source or derivation PMID/ref
SU Rifr Spontaneous derivatives from USDA 110 6330038
JH Rifr Spontaneous derivatives from USDA 110 isolate 1785926
110spc4 Spcr Spontaneious in USDA3Ib110
Bj4503 fixNOQP fixNOQP deletion mutant of 110spc4 8626278
COX132 coxA Tn5 insertion in the gene coxA of 110spc4 8626278
Bj4526 fixN fixN deletion mutant of 110spc4 9497378
Bj4621 His6-fixN 9497378
Bj4622 H330A-His6-fixN 9497378
Bj4623 H280A-His6-fixN 9497378
Bj4624 H316V-His6-fixN 9497378
Bj4625 H420A-His6-fixN 9497378
Bj4639 fixN-His6-OQP conjugated into B. japonicum strain Bj4621 10722835
LP 3001 Spcr Spontaneous derivatives from USDA 110 ref
LP 3004 Spcr Spontaneous derivatives from USDA 110 ref
BJ110 a stable small-colony derivative of strain USDA 3I1b110 3036781
Bj168, 168 Kmr nodC insertion mutant of 110spc4 2710106
Bj369, 369 Kmr insertion mutation within the nodD1-nodA intergenic region of 110spc4 2710106
Bj340, 340 Kmr nodD1 insertion mutation of 110spc4 2710106
Bj341, 314 Kmr nodD1 insertion mutation of 110spc4 2710106
Δ370 Kmr nodD2 deletion mutation of 110spc4 2710106
Δ329 Kmr nodD1-D1 deletion mutation of 110spc4 2710106
Δ321 Smr nodIJ deletion mutation of 110spc4 2710106
Δ307, Δ308, Δ309 Kmr "nod box" deletion mutation of 110spc4 2710106
Δ403, Δ408 Smr "nod box" deletion mutation of 110spc4 2710106
110rif15 Rifr derivatives of the wild-type strain 3I lb110
TAL102, RCR3427, 61A89, or SEMIA 5032 USDA 110 (also called 3I1b110, TAL102, RCR3427, 61A89, or SEMIA 5032) 11055970
E1 Fix+ Kanr/Strr Tn5 ca. 350 bp upstream nifDK, derivative of USDA 3I1b110 2985537
F4 Kanr/Strr pSUP201nifJ::Tn5-cointegrate in nifD, derivative of USDA 3I1b110 2985537
45K Tetr Kanr/Strr pSUP202nifH with Tn5 ca. 500 bp upstream nifH, derivative of USDA 3I1b110 2985537



個人用ツール
関連サービス